Shi Chen Ph.D.
Chu-Tian Professor, School of Pharmaceutical Sciences
Wuhan University, Wuhan 430071
China
Email:shichen@whu.edu.cn
Tel: 86-27-68753391
Education, and Research Positions
Harvard University,Department of Cell Biology; Chemistry and Chemical Biology Boston, MA, USA
Research Fellow, advisor: Robin Reed; co-advisor: Gregory L. Verdine Jul 2007 – May 2010
Massachusetts Institute of Technology, Department of Chemistry Cambridge, MA, USA
Postdoctoral Fellow, advisor: Sarah O’Connor Mar 2005 - Jun 2007
Shanghai Jiaotong University, School of Life Science and Technology Shanghai, China
Ph.D. in Biochemistry and Molecular Biology, advisor: Zixin Deng Sep 2000 - Oct 2004
Korea Advanced Institute of Science and Technology Daejeon, Korea
Department of Chemical & Biomolecular Engineering
Visiting Ph.D. Graduate Student, advisor: Sang Yup Lee Mar 2001 - Aug 2002
Huazhong Agriculture University, School of Life Science and Technology Wuhan, China
B.S. - M.S. in Biochemistry and Molecular Biology, advisor: Zixin Deng Sep 1994 - Jul 2000
Honors
2012 Young Talent 1000, China
2011 Chu-Tian Professor, Hubei Province, China
2008 National Natural Science Award (second grade), China
2007 Global Science Top 10, Scientific American (China)
2006 Shanghai Graduate Research Excellence Award,ShanghaiCitygovernment
2006 Tan Kah Kee Young Inventors’ Award (Shanghai),Tan Kah Kee International SocietyMerit Award
2004 1stPlaceScienceand TechnologyProgress Award, ShanghaiCitygovernment
2004 Shanghai governmentTop 11 New Era Outstandings
2004 Tan Kah Kee Young Inventors’ Award (Shanghai),Tan Kah Kee International SocietySilver Award
2004 Life ScienceExcellenceAward, Shanghai, MEIJI Dairies Corp(Japan)
2004 Shanghai - Unilever Research & Development Fund scholarship
Publications
1. Cao B. #, Chen C. #, DeMott M.S. #, Cheng Q., Clark T.A., Xiong X., Zheng X., Butty V., Levine S.S., Yuan G., Boitano M., Luong K., Song Y., Zhou X., Deng Z., Turner S.W., Korlach J., You D.*, Wang L.*,Chen S.*, Dedon P.C. Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences.Nature Communications, 2014, 5, doi:10.1038/ncomms4951. (*corresponding authors)
2. Chen Y., Deng Z, Jiang S., Hu Q., Liu H., Zhou S., Ma W.,Chen S.*, Zhao Y*. Human cells lacking coilin and Cajal bodies areproficient in telomerase assembly, trafficking and telomere maintenance.Nucleic Acids Research, 2014, 1-11, doi: 10.1093/nar/gku1277. (*corresponding authors)
3. Gan R., Wu X., He W., Liu Z., Wu S., Chen C., Chen S., Xiang Q., Deng Z., Liang D.,Chen S.*, Wang L*.DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks.Scientific Reports. 2014, Oct 16;4:6642. (*corresponding authors)
4. Wang L,Chen S*, Vergin KL, Giovannoni SJ, Chan SW, Demott MS, Taghizadeh K, Cordero OX, Cutler M, Timberlake S, Alm EJ, Polz MF, Pinhassi J, Deng Z, Dedon PC*. DNA phosphorothioation is widespread and quantized in bacterial genomes.Proceedings of the National Academy of Sciences of the United States of America.2011 Feb;108 (7):2963-2968.(*corresponding authors)
5.Wang L.#,Chen S.#, Xu T., Taghizadeh K., Wishnok J., Zhou X., You D., Deng Z,Dedon P. Phosphorothioation of DNA in bacteria bydndgenes.Nature Chemical Biology, 2007,Nov;3(11):709-710.(#co-first author)
Comments:
(1)Scientific American (China), Discovered the sixth element (sulfur) in DNA. 2007 Global Science Top10,
2008, Jan, 7-8
(2) Fritz Eckstein.NatureChemicalBiology, News and Views, Phosphorothioation of DNA in bacterial. 2007,
Nov;3(11), 689-690.
(3) Carmen Drahl.Chemical & Engineering News. DNA Backbone Swaps In Sulfur –Common artificial
DNA modification also occurs in nature. 2007, Oct22:p14.
(4) Felix Cheung.Nature China.DNA chemistry: Sulphur degradation. 2007, Oct 24:
doi:10.1038/nchina.2007.223
6.Chen S.*, Wang L., Deng Z*. Twenty years hunting for sulfur in DNA. Protein & Cell, 2010, Jan; 1(1): 14-21
7.Chen S.*, Galan M. C.*, Carla Coltharp, O'Connor S. E. Redesign of a central enzyme in alkaloid biosynthesis.Chemistry & Biology, 2006 Nov; 13(11): 1137-41. (*co-first author)
8.Chen S, Huang X, Zhou X, Bai L, He J, Jeong KJ, Lee SY, Deng Z. Organizational and mutational analysis of a complete FR-008/candicidin gene cluster encoding a structurallyrelated polyene complex.Chemistry &Biology,2003 Nov;10(11):1065-76.
9.Chen S.*, Mao X.*, Shen Y., Zhou Y., Li J., Wang L., Tao X., Yang L., Wang Y., Zhou X., Deng Z., Wei D. A tailoring P450 monooxygenase gene for FR-008/candicidin biosynthesis.Applied and Environmental Microbiology, 2009, Mar; 75(6): 1778-81(*co-first author)
10.Chen S., Zhou X.F., Jeong H.Y., Choi K.R., Lee S.Y., Deng Z. Sequence analysis of a region upstream of antibiotic FR-008 Gene cluster.Journal of Agricultural Biotechnology,2005, Mar;12(3): 404-405.
11. Wang L,Chen S, Xiao X, Huang X, You D, Zhou X, Deng Z.arsRBOCTarsenic resistance system encoded by linear plasmid pHZ227 inStreptomycessp.StrainFR-008.Applied and Environmental Microbiology, 2006 May; 72(5): 3738–3742.
12.Mao X.,Chen S.,Shen Y., Wei D, Deng Z.Effect of copper sulfate on biosynthesis of FR-008/Candicidin complex production inStreptomycessp.World Journal of Microbiology and Biotechnology, 2011, Jan, published online,DOI:10.1007/s11274-011-0665-y
13. Mao X., Yang L.,Chen S., Shen Y., Wei D., Deng Z. Influence of glucose feeding strategy on fed-batch cultures of a novel FR-008/candicidin derivatives CSl03 by a mutant of Streptomyces sp. FR-008.Industrial Microbiology, 2009, Apr; 39(2): 1-6.
14.Xu T., Liang J.,Chen S., Wang L., He X., You D., Wang Z., Li A., Xu Z., Zhou X., Deng Z. DNA phosphorothioation inStreptomyces lividans: Mutational analysis of the dnd locus. BMC Microbiology. 2009 Feb 20;9(1):41.
15.ZhouY., LiJ.,Zhu J.,ChenS., BaiL., ZhouX., WuH., DengZ.Incomplete beta-Ketone Processing as a Mechanism for Polyene Structural Variation in the FR-008/Candicidin Complex.Chemistry &Biology,2008,June;15(6):629-38.
16.Mao X., Shen Y., Yang L.,Chen S., Yang Y., Yang J., Zhu H., Deng Z., Wei D. Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant ofStreptomycessp. using statistical experimental methods.Process Biochemistry, 2007,May; 42(5): 878-883.
17.Zhou Y., Meng Q., You D., Li J.,Chen S., Ding D., Zhou X., Zhou H., Bai L., Deng Z. Selective removal of the aberrant extender unitsby a type II thioesterase for the efficient FR-008/candicidin biosynthesis inStreptomycessp.FR-008.Applied and Environmental Microbiology,2008,Dec;74(23):7235-7242.
18.Mao X., Wang F., Zhang J.,Chen S., Deng Z., Shen Y., Wei D.The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp. FR-008.AppliedBiochemistryandBiotechnology.2009,Dec;159(3):673-86
19.YerkerN.,WuJ. X.,McCoyE., GalanC.,Chen S., O’ConnorS. E.Substrate specificity and diastereoselectivity of strictosidine glucosidase, a key enzyme in monoterpene indole alkaloid biosynthesis.Bioorganic & Medicinal Chemistry Letters, 2008,May; 18(10): 3095-3098.
20. Mao X., Shen Y., Wei D.,Chen S., Deng Z.Determination of Candicidin/FR-008 and Related Components in Fermentation Broth by RP-HPLC.Journal of Chinese Pharmaceutical Sciences,2005,Feb; 14(2):115-118.
21.Yang L.,Mao X.,Zhou W.,Chen S., Shen Y., Deng Z., Wei D.Optimization on culture process conditions for biosynthesis of novel FR-008/candicidin derivatives by a mutant of Streptomyces sp.Chinese Journal of Antibiotics, 2008,June; 33(6): 333-337.
Book chapter
Wang L., Chen S.*,Deng Z*. Phosphorothioation: an unusual post-replicative modification on the DNA backbone. In Replication,2011, June.(*corresponding author)
Patents
1.Lee SY,Deng Z,Chen S, Jeong KJ, Zhou X. Genes for synthesis of FR-008 polyketides.
United States Patentnumber:7,595,156Granted
2.Lee SY,Deng Z,Chen S, Jeong KJ, Zhou X.Gene participating in synthesis of FR-008 polyketide
Japanese patentpublicationnumber:2005-124566 (JP 2005124566 A)Granted
3.Chen S, Zhou X,Deng Z,Jeong KJ,Lee SY. Genes for synthesis of FR-008 polyketides.
Korean patentapplication number:1005496900000Granted
4. Deng Z,Chen S,Zhou X. Nonmycosamine and deoxymycosamine FR-008 polyketide derivatives.
Chinese patentnumber:200310108745.1 Granted
5. Deng Z,Chen S, Zhou X, Huang X. FR-008 Polyene antibiotic complex.
Chinese patentnumber:200310108746.6 Granted
6. Deng Z, Wang L,Chen S, You D, Xiao X, Huang X. An arsenic resistance gene cluster from linear plasmid
pHZ227.
Chinese patentapplication number:200510030980.0Granted
7. Shen Y, Wei D, Mao X, Yang L, Zhou W, Deng Z,Chen S, Zhou X. Decarboxyl FR-008 polyketide derivatives
and its application.
Chinese patentapplication number:200710037060.0 Granted
8.Lee SY,Deng Z,Chen S, Jeong KJ, Zhou X. Genes for synthesis of FR-008 polyketides.
France patentapplication number:FR 0404055Published
9.Deng Z,Chen S, Zhou X,Lee SY, Jeong KJ. Gene cluster responsible for FR-008 antibiotic biosynthesis.
Chinese patentapplication number: 200310108110.1 Published
10. Shen Y, Wei D, Mao X, Yang L, Deng Z,Chen S. An optimized media for Streptomyces and its application.
Chinese patentapplication number:200610025713.9 Published
11.Shen Y, Wei D, Mao X, Deng Z,Chen S. A fermentation and production process for aromatic polyene
antibiotics.
Chinese patentapplication number:200810204283.6 Published
